Long Range PCR Master Mix, 2x
- Biotechrabbit
- BR0300400
- Na skladě
- 3 250,00Kč
-
3 188,00Kč
Dostupné možnosti
Dopravné a balné
Transportní
podmínky
cena se připočítává k položkám v objednávce
mražené na suchém ledu (dry
ice) 890
Kč bez DPH na zásilky v celkové ceně do 45 000 Kč bez DPH, nad tuto hodnotu je
dopravné a balné zdarma
Features
- Optimized Long Range PCR Master Mix for minimal hands-on and fast setup
- Mix of pure polymerases and highest quality dNTPs for high yield and short cycle times
- Increased fidelity for accurate amplification of GC-rich templatess
Applications
- High throughput, long-range PCR up to 40 kb
- Amplification of GC-rich templates
Catalog #
Size
Package Content
BR0300401
100 rxn of 50 µl
2 × 1.25 ml Long Range PCR Master Mix
500 µl 5× PCR EnhancerBR0300402
500 rxn of 50 µl
10 × 1.25 ml Long Range PCR Master Mix
1.5 ml 5× PCR EnhancerThe biotechrabbit Long Range PCR Master Mix is a perfect choice for a fast reaction set up for long PCR without the need for calculation, multiple pipetting steps and buffer optimization. It is designed for PCR amplifications of up to 30kb size DNA targets. The Master Mix works well on GC rich templates and amplifies DNA with a higher fidelity than that of Taq DNA Polymerase.
The Long Range PCR Master Mix contains a blend of thermophilic polymerases, highest quality dNTPs and optimal PCR reaction buffer components, thus only the template and PCR primers have to be added. The final reaction volume should be reached with the pure PCR grade water.
For the most demanding applications the supplied PCR additive 5x PCR Helper can be used. It is not necessary to use for every reaction, but in many cases it helps to achieve even better results with templates having high GC content and complex structures.
Long Range PCR Master Mix produces a mixture of A tailed and blunt ended PCR products, it is advisable to blunt them before cloning into the blunt ended vector.